156 The development of plant-based pharmaceutical substance for treatment of seborrheic dermatitis

Dermatological diseases of the scalp significantly affect life quality people. According to statistical data, seborrheic dermatitis (SD) is most common dermatological disease in more than 50% population all over world. The medicines for treatment SD are synthetic antimicrobial substances with high efficiency, but their regular use changes a biodiversity microflora and determines subsequent resistance. Therefore, aim study was development plant-based pharmaceutical substance SD. objects were M. alternifolia essential oil (TTO), 1,8-cineol (eucalyptol) α-(-)-bisabolol. Components tested against bacterial strains as S. epidermidis ATCC 14990, aureus 29213, C. albicans 10231 involved pathogenesis Micro-broth dilution assay used determine minimum inhibitory concentrations (MICs) compounds using resazurin cell viability method. MICs values agents different varied from 1.25 40.00 mg/mL compared benzalkonium chloride control. lowest MIC obtained TTO, eucalyptol bisabolol – 1.25, 2.50, mg/ml, respectively. effects combinations selected reference evaluated by checkerboard assay. fractional concentration index (FICI) each double combination calculated. Clear synergistic activity observed TTO FICI less 0.5. Other have additive effect. α-(-)-bisabolol exhibited on strains. Clinical dermatology evaluation indicated that added shampoo base didn’t during washing. developed has an interest medical shampoos.